If I understand correctly, the beam is a single structural shape simply supported on both ends by the floors that are considered pin-supports. One of these supports can be moved higher while the other holding constant, as thus, the question is - will the deflection change?
The answer is "Yes", because the physical beam length (along the slope) has changed, and the magnitude of deflection is direct proportional to the beam length.
The calculation method is identical to the ususl beam deflection calculation, however, the calculation shall be performed on the beam along the inclined axis, that is, the length of the beam to be input into the equation equals the horizontal span length (between supports) divided by the cosine of the angle of the incline ($L' = L/cos \theta$; Also, the gravity load needs to break into component forces -force acting normal to the beam ($N$), and force acting along the beam ($S$), note that the latter force is ignored in general practice, so the force to be input to the equation equals $N = Pcos \theta$.
Hope this answers your question.
Verification:
In order to evaluate the deflection of an inclined beam, we need to work on its own (local) coordinate system ($x$, $y$), so we need to break the force into the components - normal force ($N$), and thrust ($S_f$), and to calculate the effective length of the inclined beam ($L'). These steps are shown in the sketch below.
(*) Note, for simplicity, let's assume only the normal force causes the beam to deflect. To be exact, the thrust will increase the magnitude of the deflection to a small extent, which is usually ignored in practice, except that deflection caused by the normal force is rather large.
The calculation of the deflection is identical to the beam in horzontal:
$\Delta = \dfrac{Pcos \theta (L/cos \theta)^3}{48EI} = \dfrac {PL^3}{48EI cos^2 \theta}$
Finally, the true deflection measured in the Global Coordinate system ($X$, $Y$) is:
$\delta = \Delta cos \theta = \dfrac {PL^3}{48EI cos^2 \theta} cos \theta = \dfrac {PL^3}{48EI cos \theta}$
Now let's set $\theta = 0$, which is the condition of a horizontal beam:
$\delta = \dfrac {PL^3}{48EI cos 90^o} = \dfrac {PL^3}{48EI}$. The exact form of the deflection equation for a horizontal beam, thus, we recognize the influence of the angle of the incline.
For your case with 3 loads and the equation of the deflection could be unknown to you, you may utilize the tables below to calculate the deflection for each loading case shown, then combine the results at the points of interest through the method of "superposition".